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Table 2 Prevalence in monoclonal culture samples (n = 20) of mutations detected by traditional PCR and sequencing (gold standard) and real-time PCR, and sensitivity and specificity of realtime PCR assay.

From: A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR

  Prevalence Real-time PCR
PCR Real-time PCR Sensitivity Specificity
Tyr86 5% 5% 1/1 (100%) 19/19 (100%)
Phel84 65%   12/13 (92%) 7/7 (100%)
Cysl034 0% 0% 0/0 (--) 20/20 (100%)
Asp 1042 10% 10% 2/2 (100%) 18/18 (100%)
All mutations correct -- -- 15/16 (94%) 64/64 (100%)