Table 2 Prevalence in monoclonal culture samples (n = 20) of mutations detected by traditional PCR and sequencing (gold standard) and real-time PCR, and sensitivity and specificity of realtime PCR assay.
From: A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR
| Â | Prevalence | Real-time PCR | ||
|---|---|---|---|---|
PCR | Real-time PCR | Sensitivity | Specificity | |
Tyr86 | 5% | 5% | 1/1 (100%) | 19/19 (100%) |
Phel84 | 65% | Â | 12/13 (92%) | 7/7 (100%) |
Cysl034 | 0% | 0% | 0/0 (--) | 20/20 (100%) |
Asp 1042 | 10% | 10% | 2/2 (100%) | 18/18 (100%) |
All mutations correct | -- | -- | 15/16 (94%) | 64/64 (100%) |