A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR

Malaria Journal

Table 3 Genotype results of DNA from pre-culture and post-culture DNA by real-time PCR

Pre-culture genotype results	Post-culture genotype results
Pre-culture genotype results	Codon		86			184			1042
		Amino Acid	Asn	Tyr	His	Tyr	Tyr & Phe	Phe	Asn	Asn & Asp	Asp
	86	Asn	64	--	1
		Tyr	1	2
		His	--		--
	184	Tyr				27	1	2
		Tyr & Phe				2	4	3
		Phe				3	--	27
	1042	Asn							60	--	3
		Asn & Asp							1		1
		Asp							--	--	3

Data for codon 1034 was omitted because no mutant or polyclonal infections were detected in the 68 paired samples.

ISSN: 1475-2875