Specimen | HOLA | PCR 1e | PCR 2e |
|---|
NK5a | SS | SS | SS |
NK6 | SS | X | SS |
NK7 | RR | RR | RR |
NK8 | SS | SS | SS |
Kenya 1b | SS | X | SS |
Kenya 2 | SS | X | SS |
Kenya 3 | RS | X | RR |
Thyolo 7c | SS | SS | SS |
Thyolo 33 | SS | SS | SS |
Thyolo 34 | SS | X | SS |
Thyolo 64 | SS | X | SS |
Thyolo 75 | SS | X | SS |
RSPd | RR | RR | RR |
- aSpecimens labelled NK collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo, Kenya on 05/2004, DNA extracted by Ballinger-Crabtree method [15] on 09/2004. bSpecimens labelled Kenya collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo Bay, Kenya on 05/2004 DNA extracted by Livak method [14] on 08/2004. cSpecimens labelled Thyolo collected by Philimon Tambala and Bill Hawley from Thyolo, Malawi on 01/1995, DNA extracted by Ballinger-Crabtree method [15] on 09/1997. dSpecimen from RSP colony. Abbreviations: SS, homozygous susceptible. RR, homozygous resistant. RS, heterozygous. e Conditions for the PCR reactions were identical.
