Skip to main content

Advertisement

Table 2 Double blind trial of HOLA approach versus conventional PCR

From: A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae

Specimen HOLA PCR 1e PCR 2e
NK5a SS SS SS
NK6 SS X SS
NK7 RR RR RR
NK8 SS SS SS
Kenya 1b SS X SS
Kenya 2 SS X SS
Kenya 3 RS X RR
Thyolo 7c SS SS SS
Thyolo 33 SS SS SS
Thyolo 34 SS X SS
Thyolo 64 SS X SS
Thyolo 75 SS X SS
RSPd RR RR RR
  1. aSpecimens labelled NK collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo, Kenya on 05/2004, DNA extracted by Ballinger-Crabtree method [15] on 09/2004. bSpecimens labelled Kenya collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo Bay, Kenya on 05/2004 DNA extracted by Livak method [14] on 08/2004. cSpecimens labelled Thyolo collected by Philimon Tambala and Bill Hawley from Thyolo, Malawi on 01/1995, DNA extracted by Ballinger-Crabtree method [15] on 09/1997. dSpecimen from RSP colony. Abbreviations: SS, homozygous susceptible. RR, homozygous resistant. RS, heterozygous. e Conditions for the PCR reactions were identical.