Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1

Malaria Journal

Table 1 Primers used for genotyping P. vivax parasites.

Gene	Primer	Sequence^a	mM Mg^{2+ b}		Annealing °C ^b		Cycles^b
			1^st	2^nd	1^st	2^nd	1^st	2^nd
*Pvcs*	VCS-OF	ATGTAGATCTGTCCAAGGCCATAAA	1		58		25
	VCS-OR	TAATTGAATAATGCTAGGACTAACAATATG	1		58		25
	VCS-NF	GCAGAACCAAAAAATCCACGTGAAAATAAG		1		62		30
	VCS-NR	CCAACGGTAGCTCTAACTTTATCTAGGTAT		1		62		30
*Pvmsp1* F1	VM1-O1R	CCACTCCATGAAACTGAAGTGTTTA	2		68		25
	VM1-N1F	CGATATTGGAAAATTGGAGACCTTCATCAC	2	2	68	68	25	30
	VM1-N1R	CTTTTGCGCCTCCTCCAGCTGGCTCGTGT		2		68		30
*Pvmsp1* F2	VM1-O2F	GATGGAAAGCAACCGAAGAAGGGAAT	1		50		25
	VM1-O2R	AGCTTGTACTTTCCATAGTGGTCCAG	1	1	50	64	25	35
	VM1-N2F	AAAATCGAGAGCATGATCGCCACTGAGAAG		1		64		35
*Pvmsp1* F3	VM1-3F	CAAGCCTACCAAGAATTGATCCCCAA	1		66		42
	VM1-3R	ATTACTTTGTCGTAGTCCTCGGCGTAGTCC	1		66		42

^a Sequences are provided 5'- to 3'-end.p
^b The two columns indicate conditions for the primary and secondary amplification reactions.

ISSN: 1475-2875