Rapid screening for glucose-6-phosphate dehydrogenase deficiency and haemoglobin polymorphisms in Africa by a simple high-throughput SSOP-ELISA method

Table 2 HbB and G6PD genotype frequencies for children living in a Tanzanian village and analysed by the SSOP-ELISA method.

HbB Genotype	Numbers (87)^a	G6PD Genotype	Numbers (84)^a	68 Status	126 Status	Deduced Phenotype^b
		68–126
AA	75 (86%)	VV-NN^c	51 (61%)	Wildtype	Wildtype	G6PD B
SS	0	VV-DD^d	8 (10%)	Wildtype	Mutant	G6PD A
AS	12 (14%)	M-D^e	6 (7%)	Mutant	Mutant	G6PD A-
AC	0	VM-ND	10 (12%)	Single Mutant	Single Mutant	G6PD A
CC	0	VV-ND	8 (9%)	Wildtype	Single Mutant	G6PD A
SC	0	VM-DD	1 (1%)	Single Mutant	Mutant	G6PD A
No PCR-product	3	No PCR-product	6

^a: Numbers refer to the remaining individuals from the field with amplified PCR-product
^b: Phenotype of the G6PD trait was not measured, but deduced based on the genotype
^c: Out of the 51 individuals, 30 were males with the V-N genotype
^d: Out of the 8 individuals, 5 were males with the V-D genotype
^e: All 6 individuals were males hemizygote for M-D genotype

ISSN: 1475-2875