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Figure 1 | Malaria Journal

Figure 1

From: Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum

Figure 1

Calculation of relative efficiency of the P. falciparum glutathione reductase gene ( Pfgr ). The average Ct at each dilution was calculated for both Pf-β-actin1 and the glutathione reductase gene, Pfgr, and Ct, Pfgr-Ct, P-β-actin1 was determined (a). Plots of the log DNA dilution versus ΔCt were made (b) and a slope was calculated from this (c). The absolute value of this slope (m) was 0.0286 (< 0.1) (d), reflecting optimal relative efficiency.

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