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Table 2 Real-time quantitative PCR primers

From: Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum

Gene Primer Sequence (5' 3') Concentration (nm) Amplicon size (bp)
Pf-β-actin1 sense GGA CAC ATA TTG TGC CTG C 300 90
  antisense CTC CAC TAT CTA ACA CAA TAC C 300  
Pf-mdr1 sense CAA GTG AGT TCA GGA ATT GGT AC 300 230
  antisense GCC TCT TCT ATA ATG GAC ATG G 300  
Pf-atp6 sense GCT GCA TTC ATT AGT TTC GTG 300 126
  antisense GCC ATA CAC CTA CGG CAG C 600  
Pf-tctp sense CAA ATG ATG AAG TAT GTT CCG 300 55
  antisense GGT ACT TCA AAT GGA TCT TGT TG 600  
Pf-gpx sense CGT CGA TAA AAA TGG AGA AGT TG 300 56
  antisense CTA ACG GGT TTG TTT TGG GTG 600  
Pf-gr sense GCA GTG GCC TTA AAA ATG AAT G 300 68
  antisense GCT GTA GGA TGT ATA GGT ATG G 300  
Pf-gst sense GAT GCA AGG GGT AAA GCT G 300 150
  antisense GGG TAC TTG CTC AAA AGG AG 600  
Pf-sod1 sense GAT TAC AGA AAT GAC AGA GCA TC 300 56
  antisense CAA TTT ACT AGG TTC CAC CAA G 600  
Pf-trx1 sense CAT ATG TAG GAA GAG AAG CTC C 300 53
  antisense ATC TGC AAA AAC TGC TTC AGC C 600  
Pf-trx2 sense CGC TAG TGA CAA AGA AGG C 300 60
  antisense ACA AAC AAC AGT ATT TCT GAC C 600