Figure 3

Histidine pull-down assays of GST-4.1R recombinant proteins. A) 6His-Pf J bound specifically to GST-10 kDa in a histidine pull-down assay. The interaction was dose-dependent and reliant on native GST-10 kDa protein conformation. M, erythrocyte membrane marker; -ve, negative control pull-down using 5 μg heat denatured GST-10 kDa and +, assays indicating the dose-dependent nature of the interaction (+/++/+++ ≈ 150 ng/400 ng/800 ng GST-10 kDa respectively). B) 6His-Pf J (~800 ng) did not bind specifically to ~6 μg GST-16, 22 and 30 kDa proteins. 1, purified GST-4.1R domain; 2, histidine pull-down using heat denatured recombinant protein and 3, pull-down using non-denatured protein.