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Table 1 Oligonucleotide primers

From: The 10 kDa domain of human erythrocyte protein 4.1 binds the Plasmodium falciparum EBA-181 protein

Domain

PCR primer (5'-3')

RE

10 kDa

F: catgggatcc tggaagaaaaagagagaaag

Bam H I

 

R: catgctcgag tcagggtgagtgagtggataag

Xho I

16 kDa

F: catgggatcc tttcgatacagtggccggact

Bam H I

 

R: catgctcgag tcatgcttctgtgggctctggct

Xho I

22 kDa

F: catgggatcc ttccgaactcttaacatcaatgggcaaa

Bam H I

 

R: catgctcgag tcactcatcagcaatctcggtctcc

Xho I

30 kDa

F: catggaattc atgcactgcaaggtttctttgt

Eco R I

 

R: catgctcgag tcatttggatcctagcgcaag

Xho I

Pf J

F: catgcatgcatatg cctgaagtagttccacaagaa

Nde I

 

R: catgggatcc ttatgcactttcacctccccc

Bam H I

  1. Forward (F) and reverse (R) primer sequences were designed for amplifying the different human 4.1R domain sequences and P. falciparum 4.1R binding sequence (Pf J) in EBA-181. Endonuclease recognition sequences are underlined and the corresponding restriction enzyme (RE) indicated.