Table 1 Characteristics of primers used in nested PCR on P. falciparum DHFR. Lower case denotes nucleotide mismatch.
From: PCR detection of Plasmodium falciparum in human urine and saliva samples
Reaction | Primer | Nucleotide location | Product size (bp) |
|---|---|---|---|
Nest I | U1: 5'-GGAAATAAAGGAGTATTACCATG-3' | 121..143 | |
U2: 5'-TAAGGTTCTAGACAATATAACA-3' | 393..372 | 273 | |
Nest II | U3: 5'-GAAATGTAaTTCCCTAGATATGgAATATT-3' | 144..172 | |
U4: 5'-ATTTATCCTATTGCTTAAAGGT-3' | 372..351 | 229 | |
Nest I | M1: 5'-TTTATGATGGAACAAGTCTGC-3' | 3..18 | |
M5: 5'-AGTATATACATCGCTAACAGA-3' | 645..625 | 643 | |
Nest II | F: 5'-GAAATGTAATTCCCTAGATATGgAATATT-3' | 144..172 | |
M4: 5'-TTAATTTCCCAAGTAAAACTATTAGAgCTTC-3' | 469..439 | 326 |