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Table 1 Characteristics of primers used in nested PCR on P. falciparum DHFR. Lower case denotes nucleotide mismatch.

From: PCR detection of Plasmodium falciparum in human urine and saliva samples

Reaction Primer Nucleotide location Product size (bp)
Nest I U1: 5'-GGAAATAAAGGAGTATTACCATG-3' 121..143  
  U2: 5'-TAAGGTTCTAGACAATATAACA-3' 393..372 273
Nest II U3: 5'-GAAATGTAaTTCCCTAGATATGgAATATT-3' 144..172  
  U4: 5'-ATTTATCCTATTGCTTAAAGGT-3' 372..351 229
Nest I M1: 5'-TTTATGATGGAACAAGTCTGC-3' 3..18  
  M5: 5'-AGTATATACATCGCTAACAGA-3' 645..625 643
Nest II F: 5'-GAAATGTAATTCCCTAGATATGgAATATT-3' 144..172  
  M4: 5'-TTAATTTCCCAAGTAAAACTATTAGAgCTTC-3' 469..439 326