Table 2 Amino acid variations observed in PfCRT protein in 33 Cambodian isolates and laboratory strains within the phylogenic groups determined by Bayesian analysis. Composition of groups are as follows (laboratory clones and field isolates identified in [18]: group 1: 608, 792, 3D7, HB3; group 2: 738; group 3: 602, 613, 634, 702, 734, 736, 770; group 4: 722, 742; group 5: Dd2, W2, 536, 572, 631, 637, 643, 647, 654, 665, 666, 671, 683, 685, 691, 693, 716, 717, 719, 739, 747, 749, 794.
| Â | Residue numbers | Â | Â | Â | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
groups | 74 | 75 | 76 | 144 | 148 | 194 | 220 | 271 | 326 | 333 | 356 | 371 | N | IC50 CQ (nM) | IC50 FQ (nM) |
Group 1 | M | N | K | A | L | I | A | Q | N | T | I | R | 4 | 19.88 (11.4–33.7) | 26.59 (7.5–57.6) |
Group 2 | I | D | T | A | I | T | S | E | N | S | I | R | 1 | 156.8 | 37.3 |
Group 3 | I | D | T | F | I | T | S | E | N | S | I | R | 7 | 95.51 (33.2–169.2) | 26.34 (15.7–37) |
Group 4 | I | E | T | A | L | I | S | E | N | T | I | I | 2 | (91.8, 466.7) | (38.1, 120.2) |
Group 5 | I | E | T | A | L | I | S | E | S | T | T | I | 22 | 149.17 (58.1–674) | 39.13 (7.5–115) |