Figure 1

Detecting development of oocysts in Anopheles midguts. (A) Abdomen and dissected midgut of an infected A. stephensi mosquito with green fluorescent P. berghei. Note that single oocysts can be detected in the intact mosquito, while multiple oocysts give a blurred signal. (B) Oocyst derived fluorescence detected in a well infected living A. albimanus mosquito (left) and an isolated midgut (right) 26 days post infection. In the mosquito the fluorescence appears blurred due to the opaque nature of the abdomen's chitin. (C) Four representative photographs from midguts of infected A. albimanus, A. gambiae and A. stephensi mosquitoes. The days after the infectious blood meals are indicated.