Figure 3

Infectious sporozoites in A. albimanus. (A) Isolated salivary gland of an infected A. albimanus mosquito at 26 days post infection. Sporozoites are shown in the left panel, while the movement occurring over 200 seconds is shown in the middle panel. This panel represents the projected standard deviation between time frames of a movie that spans 200 seconds with one image taken every 2 seconds. The insets show a typical back-and-forth moving sporozoite (arrowheads indicate ends of sporozoite). The time between frames is indicated in seconds. The right panel shows a merge of the static sporozoites (green) and the movement (red). See also movie 3. (B) An isolated sporozoite glides on a glass surface in the presence of 5% foetal calf serum. The asterix indicates the apical end of the sporozoites at 0 seconds, while the arrowhead indicates the apical end at the respective time frame. Time between frames is indicated in seconds. Scale bar: 10 μm. (C) Blood smear at 13 days after the injection of 2.000 salivary gland sporozoites shows an early trophozoite.