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Table 2 Oligonucleotide sequences and hybridization temperatures (HT) used for genotyping transporter polymorphisms.

From: Lack of association between putative transporter gene polymorphisms in Plasmodium falciparum and chloroquine resistance in imported malaria isolates from Africa

Genea Predicted products Position AA change Primer (sense) Primer (antisense) HT (°C)
pfcrt Putative transporter 76 K-T 5'-TTTAGGTGGAGGTTCTTG-TC-3' 5'-ATAAAGTTGTGAGTTTCGGA-3' 59
   220 A-S 5'-TTATACAATTATCTCGAAGCAG-3' 5'-CATGTTTGAAAAGCATACAGGC-3' 59
pfmdr1 ABC transporter 86 N-Y 5'-AGAGAAAAAAGATGGTAACCTCAG-3' 5'-ACC-ACAAACATAAATTAACGG-3' 51
G7 (PF13-0271) ABC transporter 1390 &1 5'-GCTAAAGAAAAGGATCCGAACA-3' 5'-CAACCTTTTCTCCATTTTCAAT-3' 50
G25 (PF14-0679) Sulfate permease Intron G-A 5'-TATGGGAGGTGCTGAATGTG-3' 5'-TCGTTATCTTCGAAATTGTAGCC-3' 61
G30 (PF14-0292) GTPase Intron C-G 5'-AATTGCAAAGGGAAGGAAGG-3' 5'-TTGGGTACACGCACGTTAAG-3' 61
G49 (PF08-0078) ABC/ATPase 146 Q-E 5'-GGAAGGCATTATAGCAAACC-3' 5'-AGTCGTTTTCCCACATCCA-3' 56
   1046 L-I 5'-TGAACTTATTGAGACCGGTGAA-3' 5'-ACGCACTTTTCACCTCCA-3' 56
   1116 L-I 5'-TGAACTTATTGAGACCGGTGAA-3' 5'-ACGCACTTTTCACCTCCA-3' 56
G54 (PF14-0260) Membrane protein 141 Y-Y 5'-TTGGATCAGACATTACCATC-3' 5'-GGATATTGTTCCTCAAGCTCCT-3' 56
   144 T-T 5'-TTGGATCAGACATTACCATC-3' 5'-GGATATTGTTCCTCAAGCTCCT-3' 56
G55 (PF14-0133) ABC transporter Intron &2 5'-ATAATGCATACATAACCTTACC-3' 5'-TCCCATTTATGTAATATGAAC-3' 51
G70 (PFL0620c) Choline transporter 105 E-K 5'-TTGATGCGTGTGTATTGATA-3' 5'-ATGTGAACCACCTTCTGGA-3' 56
  1. aPlasmoDB (version 4) gene identifiers are shown in brackets
  2. &1 represents a trinucleotide insertion
  3. &2: microsatellite polymorphism located 700 bp downstream of the stop codon
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Malaria Journal

ISSN: 1475-2875

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