Table 2 Oligonucleotide sequences and hybridization temperatures (HT) used for genotyping transporter polymorphisms.
Genea | Predicted products | Position | AA change | Primer (sense) | Primer (antisense) | HT (°C) |
|---|---|---|---|---|---|---|
pfcrt | Putative transporter | 76 | K-T | 5'-TTTAGGTGGAGGTTCTTG-TC-3' | 5'-ATAAAGTTGTGAGTTTCGGA-3' | 59 |
220 | A-S | 5'-TTATACAATTATCTCGAAGCAG-3' | 5'-CATGTTTGAAAAGCATACAGGC-3' | 59 | ||
pfmdr1 | ABC transporter | 86 | N-Y | 5'-AGAGAAAAAAGATGGTAACCTCAG-3' | 5'-ACC-ACAAACATAAATTAACGG-3' | 51 |
G7 (PF13-0271) | ABC transporter | 1390 | &1 | 5'-GCTAAAGAAAAGGATCCGAACA-3' | 5'-CAACCTTTTCTCCATTTTCAAT-3' | 50 |
G25 (PF14-0679) | Sulfate permease | Intron | G-A | 5'-TATGGGAGGTGCTGAATGTG-3' | 5'-TCGTTATCTTCGAAATTGTAGCC-3' | 61 |
G30 (PF14-0292) | GTPase | Intron | C-G | 5'-AATTGCAAAGGGAAGGAAGG-3' | 5'-TTGGGTACACGCACGTTAAG-3' | 61 |
G49 (PF08-0078) | ABC/ATPase | 146 | Q-E | 5'-GGAAGGCATTATAGCAAACC-3' | 5'-AGTCGTTTTCCCACATCCA-3' | 56 |
1046 | L-I | 5'-TGAACTTATTGAGACCGGTGAA-3' | 5'-ACGCACTTTTCACCTCCA-3' | 56 | ||
1116 | L-I | 5'-TGAACTTATTGAGACCGGTGAA-3' | 5'-ACGCACTTTTCACCTCCA-3' | 56 | ||
G54 (PF14-0260) | Membrane protein | 141 | Y-Y | 5'-TTGGATCAGACATTACCATC-3' | 5'-GGATATTGTTCCTCAAGCTCCT-3' | 56 |
144 | T-T | 5'-TTGGATCAGACATTACCATC-3' | 5'-GGATATTGTTCCTCAAGCTCCT-3' | 56 | ||
G55 (PF14-0133) | ABC transporter | Intron | &2 | 5'-ATAATGCATACATAACCTTACC-3' | 5'-TCCCATTTATGTAATATGAAC-3' | 51 |
G70 (PFL0620c) | Choline transporter | 105 | E-K | 5'-TTGATGCGTGTGTATTGATA-3' | 5'-ATGTGAACCACCTTCTGGA-3' | 56 |