Lack of association between putative transporter gene polymorphisms in Plasmodium falciparum and chloroquine resistance in imported malaria isolates from Africa

Table 3 Association between polymorphims in pfmdr 1 and 5 putative transporter genes and chloroquine response phenotype and pfcrt 76 mutant allele of 27 P. falciparum isolates.

Gene	Predicted product	Position	AA change	Nucleotide change	IC₅₀ ^aCQ	pfcrt K76T
pfcrt	Transporter	76	K-T	AAA-ACA	P<.02	-
		220	A-S	GCC-TCC	P<.05	P<.001
pfmdr1	ABC transporter	86	N-Y	AAT-TAT	P<.05	P = .37
G25	Sulfate permease	Intron		G-A	NA	NA
G30	GTPase	Intron	-	C-G	P = .65	P = .9
G49	ABC/ATPase	146	Q-E	CAA-GAA	P = .11	P = .9
		1046	K-I	AAA-ATA	NA	NA
		1116	L-I	TTA-ATA	P = .54	P = .19
G54	Membrane protein	141	Y-Y	TAC-TAT	P = .58	P = .62
		144	T-T	ACG-ACA	P = .54	P = .63
G70	Choline transporter	105	E-K	GAA-AAA	P = .11	P = .9

^aFifty percent inhibitory concentration values : resistance value to chloroquine (CQ) corresponds to IC₅₀ > 100 nM
NA for not applicable : no SNP was found in the studied isolates and the reference strains

ISSN: 1475-2875