Figure 1From: Detection of atovaquone-proguanil resistance conferring mutations in Plasmodium falciparum cytochrome b gene in Luanda, AngolaRestriction digests for detection of pfcytb codon 268 mutations on field samples. A. 600 bp amplification product with the primer pair for wild type (TAT) detection, digested by Mph1103-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6, 8 field isolates. MWM – 100 bp molecular weight marker. B. 146 bp amplification product with the primer pair for mutation T802A (AAT) detection, digested by Dra-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker. C.173 bp amplification product with the primer pair for mutation A803C (TCT) detection, digested by Cai-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker.Back to article page