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Figure 1 | Malaria Journal

Figure 1

From: Detection of atovaquone-proguanil resistance conferring mutations in Plasmodium falciparum cytochrome b gene in Luanda, Angola

Figure 1

Restriction digests for detection of pfcytb codon 268 mutations on field samples. A. 600 bp amplification product with the primer pair for wild type (TAT) detection, digested by Mph1103-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6, 8 field isolates. MWM – 100 bp molecular weight marker. B. 146 bp amplification product with the primer pair for mutation T802A (AAT) detection, digested by Dra-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker. C.173 bp amplification product with the primer pair for mutation A803C (TCT) detection, digested by Cai-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker.

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