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Table 1 Primer sequences used for the amplification of pfcytb codon 268.

From: Detection of atovaquone-proguanil resistance conferring mutations in Plasmodium falciparum cytochrome b gene in Luanda, Angola

Primer Sequence
OD 5'-CGCAACAGGTGCTTCTCTTGT-3'
OR 5'-ACAGAATAATCTCTAGCACCAAAAATCAT-3'
WtR 5'-GGTTTACTTGGAACAGTTTTTAACAaTG-3'
802D 5'-GTTTATTTGGAATTATACCTTTATCACATCCTGATAATGCTATC-3'
802R 5'-TAAACCAGCTGGTTTACTTGGAACAGTTTTTAACATTGtt-3'
803D 5'-CCTGAATGGTACTTTCTACCAgTT-3'
  1. Small case indicates base changes to provide digest sites for restriction enzymes to distinguish wild type from mutations at codon 268. O: outer PCR; D: direct primer; R: reverse primer; Wt: wild type.
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