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Table 1 Primer sequences used for the amplification of pfcytb codon 268.

From: Detection of atovaquone-proguanil resistance conferring mutations in Plasmodium falciparum cytochrome b gene in Luanda, Angola

Primer

Sequence

OD

5'-CGCAACAGGTGCTTCTCTTGT-3'

OR

5'-ACAGAATAATCTCTAGCACCAAAAATCAT-3'

WtR

5'-GGTTTACTTGGAACAGTTTTTAACAaTG-3'

802D

5'-GTTTATTTGGAATTATACCTTTATCACATCCTGATAATGCTATC-3'

802R

5'-TAAACCAGCTGGTTTACTTGGAACAGTTTTTAACATTGtt-3'

803D

5'-CCTGAATGGTACTTTCTACCAgTT-3'

  1. Small case indicates base changes to provide digest sites for restriction enzymes to distinguish wild type from mutations at codon 268. O: outer PCR; D: direct primer; R: reverse primer; Wt: wild type.
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Malaria Journal

ISSN: 1475-2875

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