Table 2 Primer pairs and restriction enzymes used for the identification of each pfcytb codon 268 polymorphism.
Codon 268 | Type | Primer pair | Product size (bp) | Restriction enzyme | Restriction pattern | Fragment length (bp) |
|---|---|---|---|---|---|---|
Outer PCR | OD ; OR | 716 | ||||
TAT | Wild type | OD ; WtR | 600 | Mph1103-I | Cuts Wt, T802A | 570 (+30) |
AAT | T802A | 802D; 802R | 146 | Dra-I | Cuts T802A | 104 (+42) |
TCT | A803C | 803D; OR | 173 | Cai-I | Cuts A803C | 148 (+25) |