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Table 2 Standard Curves. Functions used to adjust Pyrosequencing allele frequencies for six polymorphic sites in MSP-119.

From: A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa

SNP Location Function* Parameter values
a b c d g
1644 a - dsin(2πp) -0.00497 - - 0.0292 -
1691 a+bp+cp2 - dsin(2πp) 0.00894 0.176 -0.170 0.0162 -
1699 bp - dsin(2πp) - 0.0522 - 0.0261 -
1700 bp + cp2 - dsin(2πp) - 0.0966 -0.0851 0.0219 -
1701 a+cp2 - dsin(2πp) -0.0303 - 0.133 0.0223 -
1716 -dsin(2gπp) - - - 0.0311 1.051
  1. *Where p is the allele frequency at the SNP of interest
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