Table 1 Details of primers, primer and MgCl2 concentrations and cycle conditions for the amplification of PfWARP and Pfs28 genes. Oligonucleotide positions are based on NCBI Genebank sequence PfWARP (NC_004329) and Pfs28 (L25843).
Oligonucleotide | Nucleotide sequence | Description | Primer (μM)/MgCl2 (mM) | Cycle conditions |
|---|---|---|---|---|
PfWARP_USF | GTTGTTGTATAATAAGAGAGAGAAAAATG | WARP bases 1221181 to 1221209 | 0.6/2.0 | 94°C 10 min (94°C 30 sec, 52°C 30 sec, 72°C 60 sec) 40 cycles, 72°C 5 min |
PfWARP_IR | TACATCTTATGATTTATTCTTATCACATA | WARP bases 1222057 to 1222085 | Â | Â |
PfWARP_IF | GTGGTATTATGTTTGGGTATGATATCAGC | WARP bases 1221222 to 1221250 | 0.6/1.5 | 94°C 10 min (94°C 30 sec, 52°C 30 sec, 72°C 60 sec) 25 cycles, 72°C 5 min |
PfWARP_IR | TACATCTTATGATTTATTCTTATCACATA | WARP bases 1222057 to 1222085 | Â | Â |
Pfs28_F1 | ATGAATACATATTTTAAGGTACTTCTT | Pfs28 bases 60 to 86 | 1.0/2.5 | 94°C 10 min (94°C 60 sec, 46°C 60 sec, 72°C 45 sec) 40 cycles, 72°C 10 min |
Pfs28_R650 | GAGCATACAATCAGAACGTGTGTTAGG | Pfs28 bases 680 to 709 | Â | Â |
Pfs28_F40 | CAACTTTACATAACGTTGAATAAGGCTC | Pfs28 bases 99 to 126 | 1.0/2.5 | 94°C 10 min (94°C 60 sec, 50°C 60 sec, 72°C 45 sec) 34 cycles, 72°C 10 min |
Pfs28_R630 | GCATACAATCAGAACGTGTGTTAG | Pfs28 bases 666 to 689 | Â | Â |