Table 2 Double blind trial to compare results SSOP-ELISA for kdr genotyping to results of standard multiplex PCR methods using control strains of known genotype (L, Dondotha/R70; F, VKPR; S, RSP) as individuals or in mixtures.
Test Sample | Genotype results by method | ||
|---|---|---|---|
Genotype(Strain) | SSOP-ELISA | Multiplex PCR | |
| Â | Â | East African kdr1 | West African kdr2 |
L(Dond)+F(VKPR) | L/F | L | L/X |
L(Dond)+S(RSP) | L/S | X/S | L |
F(VKPR) | F | -- | F |
field sample | L | L | L |
S(RSP) | S | S | -- |
L(Dond) | L | L | L |
F(VKPR)+S(RSP) | F/S | S | F |
L(R70)+F(VKPR) | L/F | L | L/F |
L(R70)+S(RSP) | L/S | X/S | L |
L(R70) | L | L | L |
F(VKPR) | F | -- | F |
S(RSP) | S | S | -- |