Flow cytometric analysis of eosin-5-maleimide (EMA)-labelled P. falciparum infected (iRBC) and unifected RBC (nRBC) before and after interactions with THP-1 monocytes. Trophozoite infected human group O+ red cells (1 × 106) in RPMI 1640 medium were co-incubated with TNF-α activated THP-1 monocytes (3 × 106) for 1 hour at 37°C. Following the interaction, the cell mixture was stained with EMA by incubation in the dark for one hour at room temperature. Panel A is an overlay histogram of fluorescent profile of EMA labeled iRBC before (dashed lines) and after interaction (continuous lines) for the best of the 6 experiments. Panel B is a bar graph of median channel fluorescence (MCF, ± SEM) for 6 experiments of iRBC and nRBC following the indicated treatments.