Autoradiograph of proteins from trophozoite stage iRBC ghosts from A4, C24 and ItG. At 20 hours after invasion, parasites were metabolic labelled with 50 μCi/ml [35S] methionine for 4 hours. IRBC ghosts were separated from the free parasite fraction and run on pH 4-7 IEF strips followed by 12.5% SDS-PAGE. Gels were stained with Coomassie Blue, dried and exposed to x-ray film. Marked circles show proteins with at least three fold changes in expression compared with C24. High quality tif files showing the original 2D gels are available for A4 (Additional file 8), C24 (Additional file 9) and ItG (Additional file 10) iRBC ghosts from trophozoite stages.