Representative 2D gel of parasite proteins from ItG trophozoites. ItG trophozoites were enriched 24 hours after invasion by Plasmagel floatation, the free parasite fraction was run on pH 3-10 IEF strips followed by 12.5% SDS-PAGE and the gel was stained with Coomassie Blue. Spots were excised and identified by MALDI-TOF. Solid circles indicate proteins from human source and dotted circles label proteins from parasite source. All marked protein IDs achieved significant protein scores in Mascot search results. An unannotated high quality tif file of the original gel is available (Additional file 21).