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Table 2 Giemsa smear results among 24 remaining reference readers for 35 donations subjected to external validation

From: Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency

Donor

Composite Diagnosis

Reader Agreement %

PCR Result

Reference Reader Results

Median Parasite Density (per ul)

12

Pf/Pv

13

PfPv

3Pf/Pv, 21Pf

31,222

8

Pm

50

Pm

12Pm, 2Pm/Pv, 2Pv, 2Pf, 5neg, 1 no sp

95

16

Pm

58

Pm

14Pm, 10Pf/Pm

3,183

6

Pf/Pv

63

PfPv

15Pf/Pv, 6Pf, 3Pf/Po

15,084

11

Pf/Pv

67

PfPv

16 Pf/Pv, 6Pv, 1Pf/Po, 1Pf/Pm/Pv

129,437

50

Pv

83

Pv

20Pv, 3Po, 1Po/Pv

14,895

2

Pv

83

Pv

20Pv, 4Pf/Pv

15,000

15

Pv

88

Pv

21Pv, 2Pm, 1neg

64

17

Pv

88

Pv

21 Pv, 2 Po, 1Po/Pv

1,335

49

Pv

88

Pv

21 Pv, 2 Po, 1Pm/Po/Pv

328

4

NEG

92

neg

22neg, 2 Pf

n/a

52

NEG

92

neg

22neg, 1 Pf, 1Pv

n/a

10

Pf

96

Pf

23Pf, 1Pf/Pv

1,620

20

Pf

96

Pf

23Pf, 1 no species

202

21

Pf

96

Pf

23Pf, 1Pf/Pm

165,500

22

NEG

96

neg

23neg,1Pf

n/a

1

Pf

100

Pf

24Pf

15,453

3

Pf

100

Pf

24Pf

3,542

7

Pf

100

Pf

24Pf

3,669

9

Pf

100

Pf

24Pf

340

13

Pf

100

Pf

24Pf

1,048

18

Pf

100

Pf

24Pf

301,996

19

Pf

100

Pf

24Pf

1,404

23

NEG

100

neg

24neg

n/a

44

NEG

100

neg

24neg

n/a

46

Not used*

50

Pf

12Pf, 12 neg

9

58

Not used*

58

Pv

14Pv, 4Po, 2Pm, 1Pm/Po/Pv, 1Pf/Po, 1neg, 1no sp

63

14

Not used*

63

Pv

15Pv, 4Po, 3Pf/Pv, 1Pm/Pv, 1Pm/Po/Pv

5,191

25

Not used*

67

Pf

16Pv, 2Po, 1Pf, 5neg

48

59

Not used*

71

Pv

17Pv, 1Po, 2Pf, 2neg, 1Pf/Po/Pv, 1Pf/Pm/Po

135

60

Not used*

75

Pf

18Pf, 1Po, 3neg, 2no sp

90

54

Not used*

79

Pf

19Pf, 2Pv, 1neg, 2no species

78

55

Pf* not used

83

Pf

20Pf, 1Pv, 1Po, 1Pf/Pm, 1 no species

129

5

Pf* not used

100

Pf

24Pf

2,625

24

Pf* not used

100

Pf

24Pf

10,885

  1. Pf = Plasmodium falciparum, Pv = P. vivax, Pm = P. malariae
  2. * Donation not included in the U.S. National Institutes of Health Malaria Research and Reference Reagent Resource Center (MR4) malaria slide repository either because composite diagnosis could not be determined or because slides not required for sets