Table 1 Mean IC50 values for chloroquine and pyrimethamine determined by the PicoGreen® or [3H]-hypoxanthine accumulation assay. Mean IC50 values for chloroquine and pyrimethamine were determined by the PicoGreen® or the [3H]-hypoxanthine accumulation assay for two laboratory lines of P. falciparum, 3D7 and K1. Data are the means of 3 independent determinations and standard error of the mean (s.e.m.) values are given in brackets. P-values were obtained from Student's t-test comparisons of IC50 values obtained for the two methods.
Parasite | Mean IC50 values in nM (s.e.m.) | ||
|---|---|---|---|
Drug | PicoGreen | [ 3 H]-hypoxanthine | P-value |
3D7 | |||
Chloroquine | 6.3 (0.4) | 7.2 (1.1) | 0.47 |
Pyrimethamine | 38.6 (7.6) | 47.4 (9.6) | 0.51 |
K1 | |||
Chloroquine | 141 (29.4) | 128 (7.4) | 0.69 |
Pyrimethamine | 295 (115.2) | 437 (133.4) | 0.47 |