Effect of adjuvant selection and time after immunization on TB activity. Individual and pooled rabbit anti-Pfs25 sera were diluted 1:2 and 1:8, and then tested for TB activity in mosquito membrane feeding assays as described in the legend to Figure 3. (a) data were categorized according to the adjuvant used for immunization (open circles: alum; closed circles: Montanide ISA720) while in (b) data were categorized according to number of days after initial immunization (open triangles: day 42; closed triangles: day 70). Lines represent regression of result by use of a hyperbolic equation. Four data points, which showed more than 30,000 antibody units and 100% inhibition of oocyst density per mosquito, are not shown in these figures.