An example of results from each of the PCR assays examined in this study. The example uses a paired single infection of Plasmodium falciparum and Plasmodium vivax. A. Image of P. falciparum (300-bp) and P. vivax (276-bp) positive samples after multiplex PCR, run on 2% agarose at 100 v for 1 hour. B. P. falciparum (205-bp) and P. vivax (120-bp) positive samples after 2nd round nested PCR, run on 2% agarose at 100 v for 1 hour. C. Real-time PCR melt curves of P. falciparum (T
74.5) and P. vivax (T
78). Image provided by Optical Monitor Software v.3.1.