Species identification using the real-time TaqMan assay designed by Walker et al. In this example two specimens of An. gambiae s.s., (red trace) An. arabiensis, (blue trace) An. melas (green trace), An. merus (purple trace) and An. quadriannulatus (orange trace) were tested. Part A displays the cycling of FAM-labelled probe specific for An. arabiensis. Part B displays the cycling of the VIC-labelled probe designed to be specific for An. gambiae s.s. and shows the non-specific amplification of the An. melas, An. merus and An. quadriannulatus samples.