Cell biology applications of cultured hepatocytes. (a) Light microscopy image of a hepatocyte monolayer at 24 h culture. (b) Immunofluorescence image of hepatocytes 36 h after infection with GFP-expressing Plasmodium berghei and DAPI staining. (c) Confocal image of hepatocyte stained with monoclonal antibodies to Transferrin receptor labelled with Alexa488 (Invitrogen). d) Confocal image of hepatocyte transfected with an EGFP construct using Lipofectamine reagent (Invitrogen). Bars, 20 μm.