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Figure 1 | Malaria Journal

Figure 1

From: Promoter regions of Plasmodium vivax are poorly or not recognized by Plasmodium falciparum

Figure 1

Upstream regions of P. vivax msp1 , dhfr and vir3 are not recognized by P. falciparum. A. Luciferase reporter plasmids constructed with P. vivax upstream regions. PvMSP 5' – 5' upstream region of P. vivax merozoite surface protein one; PvDHFR 5' – 5'upstream region of P. vivax dihydrofolate reductase gene; Pvvir 3 5' – 5' upstream region of P. vivax variant gene 3; Pb ef-1α IR – P. berghei elongation factor one alpha intergenic region; LUC – luciferase; DT3' – P. berghei dhfr 3' UTR; HRP2 3' – P. falciparum histidine rich protein 2 3' UTR. pE(A)b.luc.^D has the P. berghei ef-1α intergenic region. B. Transient transfection in P. falciparum. Plasmids were transiently transfected in P. falciparum, parasites kept in culture for four days and luciferase activity detected. Luciferase activity is expressed relative to pE(A)b.luc.^D used throughout this study as the positive control. Negative control (-) refers to luminescence measures of the substrate alone or plasmid with luciferase and malaria 3' UTR but no promoter. Log scale was used. Values represent the mean of at least three independent experiments done with two or three different DNA preparations. Bars represent standard deviations.

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