Figure 3From: Identification of field caught Anopheles gambiae s.s. and Anopheles arabiensis by TaqMan single nucleotide polymorphism genotypingA. Gel phenotype of standard PCR for wild type An. gambiae s.l. from western Kenya, designated as hybrids by TaqMan genotyping. Lanes 1 – 11, field samples; Lane 12, a negative control. Lane 3 shows a true field hybrid with bands at the predicted 390 and 315 bp positions; lane 6 was a nonreactive sample in standard PCR. All other lanes are An. arabiensis by standard PCR, showing a predicted 315 bp product. B. Gel phenotype of standard PCR for wild type An. arabiensis and An. gambiae from western Kenya. Lanes 1 – 4, An. arabiensis by TaqMan genotyping. Lanes 5 – 9, An. gambiae by TaqMan genotyping. Lanes 10 – 12, undetermined by TaqMan genotyping, and standard PCR yielded no amplicon. A 390 bp amplicon is predicted for An. gambiae and a 315 bp amplicon is predicted for An. arabiensis. Lane M in both figures is a 1 Kb molecular weight ladder.Back to article page