A. Gel phenotype of standard PCR for wild type An. gambiae s.l. from western Kenya, designated as hybrids by TaqMan genotyping. Lanes 1 – 11, field samples; Lane 12, a negative control. Lane 3 shows a true field hybrid with bands at the predicted 390 and 315 bp positions; lane 6 was a nonreactive sample in standard PCR. All other lanes are An. arabiensis by standard PCR, showing a predicted 315 bp product. B. Gel phenotype of standard PCR for wild type An. arabiensis and An. gambiae from western Kenya. Lanes 1 – 4, An. arabiensis by TaqMan genotyping. Lanes 5 – 9, An. gambiae by TaqMan genotyping. Lanes 10 – 12, undetermined by TaqMan genotyping, and standard PCR yielded no amplicon. A 390 bp amplicon is predicted for An. gambiae and a 315 bp amplicon is predicted for An. arabiensis. Lane M in both figures is a 1 Kb molecular weight ladder.