Table 1 Sequence of primers and probes used for Pfcrt and Pfmdr1 amplification and melting temperatures of the sensor probes of each allele
Melting temperatures (°C) of the sensor probes | ||
|---|---|---|
Sequence 5' to 3' | Wild | Mutant |
Pfcrt | ||
Forward Primer: CTTGTCTTGGTAAATGTGCTCA | ||
iLC Primer: GTTACCAATTTTGTTTAAAGTTCT | ||
Sensor Probe: TGTGTAATTGAAACAATTTTTGCTAA | 46.5 ± 0.2 | 65.3 ± 0.4 |
Pfmdr1 | ||
Forward Primer: TGTATTATCAGGAGGAACATTACC | ||
Reverse Primer: ACCACCAAACATAAATTAACGGA | ||
Sensor Probe 86: ATTAATATCATCATAAATACATG | 51.8 ± 0.3 | 56.5 ± 0.2 |
Anchor Probe 86: TCTTTAATATTACACCAAACACAGATAT | ||
Sensor Probe 184: TAAAAAATGCACGTTTGACTTTATGTATTA | 53.0 ± 0.2 | 58.7 ± 0.3 |
Anchor Probe 184: CCTTTTTAGGTTTATTTATTTGGTCAT | ||