Figure 1From: Sub-cellular localization and post-translational modifications of the Plasmodium yoelii enolase suggest moonlighting functions(A) Western blot analysis of soluble (S) and particulate (P) fractions of P. yoelii cell extract. The proteins from the two fractions were analysed on a 12% SDS-PAGE and the blot was probed for the presence of enolase (~50 kDa) and aldolase (~40 kDa). (B) Western blot of soluble (S) and particulate (P) fractions of P. yoelii merozoite extract. (C) Western blot for the detection of enolase (Pfen) associated with the (a) membrane fraction, (b) nuclear fraction and (c) cytoskeletal fraction. The proteins in the samples were analysed on a 12% SDS-PAGE and the blots were probed using r-Pfen antibodies for the presence of enolase (~50 kDa).Back to article page