Skip to main content

Advertisement

Table 1 Effect of plasma samples, cytokines and parasite products in the white blood cell aggregation assay

From: Plasmodium vivax: paroxysm-associated lipids mediate leukocyte aggregation

Expt. No. Sample % RCAI (Median) 25% 75%
1 NHP 5.80 1.6 7.3
2 PP 100 100.0 100.0
3 POPP 20.90 5.6 28.4
4 NHP+cytokines 17.20 6.7 23.5
5 NHP+PvAg 18.60 4.5 20.8
6 NHP+PvAg+ cytokines 88.50 79.8 92.4
7 NHP+RBC Ag+ cytokines 10.00 2.4 12.9
8 POPP+cytokines 18.30 7.5 22.3
9 POPP+PvAg 16.40 9.4 18.1
10 POPP+PvAg+ cytokines 77.10 64.7 79.8
11 NHP+LPS 6.10 1.9 7.2
12 NHP+LPS+cytokines 16.10 6.9 18.6
13 PP + IRS-Pv 24.90 15.6 28.4
14 PP + IRS-Pf 54.40 48.6 65.4
15 PP + CHS 17.00 14.2 19.5
  1. % RCAI: % Relative Cell Aggregation Index
  2. 25%–75%: 25th and 75th percentiles
  3. Normal human plasmas (NHP) and post paroxysm plasmas (POPP) were reconstituted with freeze-thawed extracts of P. vivax schizonts (Pv Ag), normal red cell extracts (RBC Ag), recombinant human cytokines TNF-, GM-CSF, IL-6 and IL-10 (cytokines) or E. coli lipopolysaccharide (LPS). P. vivax paroxysm plasma (PP) was pre-incubated with rabbit serum raised against P. vivax schizonts (IRS-Pv), with rabbit serum raised against P. falciparum schizonts (IRS-Pf) or with convalescent human serum (CHS) collected 4 – 6 weeks after drug cure of a P. vivax infection, from semi-immune individuals resident in a malaria-endemic area prior to being tested in the cell aggregation assay. The table provides the median values together with the interquartile range (25%–75%).