Expt. No.
|
Sample
|
% RCAI (Median)
|
25%
|
75%
|
---|
1
|
NHP
|
5.80
|
1.6
|
7.3
|
2
|
PP
|
100
|
100.0
|
100.0
|
3
|
POPP
|
20.90
|
5.6
|
28.4
|
4
|
NHP+cytokines
|
17.20
|
6.7
|
23.5
|
5
|
NHP+PvAg
|
18.60
|
4.5
|
20.8
|
6
|
NHP+PvAg+ cytokines
|
88.50
|
79.8
|
92.4
|
7
|
NHP+RBC Ag+ cytokines
|
10.00
|
2.4
|
12.9
|
8
|
POPP+cytokines
|
18.30
|
7.5
|
22.3
|
9
|
POPP+PvAg
|
16.40
|
9.4
|
18.1
|
10
|
POPP+PvAg+ cytokines
|
77.10
|
64.7
|
79.8
|
11
|
NHP+LPS
|
6.10
|
1.9
|
7.2
|
12
|
NHP+LPS+cytokines
|
16.10
|
6.9
|
18.6
|
13
|
PP + IRS-Pv
|
24.90
|
15.6
|
28.4
|
14
|
PP + IRS-Pf
|
54.40
|
48.6
|
65.4
|
15
|
PP + CHS
|
17.00
|
14.2
|
19.5
|
- % RCAI: % Relative Cell Aggregation Index
- 25%–75%: 25th and 75th percentiles
- Normal human plasmas (NHP) and post paroxysm plasmas (POPP) were reconstituted with freeze-thawed extracts of P. vivax schizonts (Pv Ag), normal red cell extracts (RBC Ag), recombinant human cytokines TNF-∝, GM-CSF, IL-6 and IL-10 (cytokines) or E. coli lipopolysaccharide (LPS). P. vivax paroxysm plasma (PP) was pre-incubated with rabbit serum raised against P. vivax schizonts (IRS-Pv), with rabbit serum raised against P. falciparum schizonts (IRS-Pf) or with convalescent human serum (CHS) collected 4 – 6 weeks after drug cure of a P. vivax infection, from semi-immune individuals resident in a malaria-endemic area prior to being tested in the cell aggregation assay. The table provides the median values together with the interquartile range (25%–75%).