Expt. No. | Sample | % RCAI (Median) | 25% | 75% |
---|
1 | NHP | 5.80 | 1.6 | 7.3 |
2 | PP | 100 | 100.0 | 100.0 |
3 | POPP | 20.90 | 5.6 | 28.4 |
4 | NHP+cytokines | 17.20 | 6.7 | 23.5 |
5 | NHP+PvAg | 18.60 | 4.5 | 20.8 |
6 | NHP+PvAg+ cytokines | 88.50 | 79.8 | 92.4 |
7 | NHP+RBC Ag+ cytokines | 10.00 | 2.4 | 12.9 |
8 | POPP+cytokines | 18.30 | 7.5 | 22.3 |
9 | POPP+PvAg | 16.40 | 9.4 | 18.1 |
10 | POPP+PvAg+ cytokines | 77.10 | 64.7 | 79.8 |
11 | NHP+LPS | 6.10 | 1.9 | 7.2 |
12 | NHP+LPS+cytokines | 16.10 | 6.9 | 18.6 |
13 | PP + IRS-Pv | 24.90 | 15.6 | 28.4 |
14 | PP + IRS-Pf | 54.40 | 48.6 | 65.4 |
15 | PP + CHS | 17.00 | 14.2 | 19.5 |
- % RCAI: % Relative Cell Aggregation Index
- 25%–75%: 25th and 75th percentiles
- Normal human plasmas (NHP) and post paroxysm plasmas (POPP) were reconstituted with freeze-thawed extracts of P. vivax schizonts (Pv Ag), normal red cell extracts (RBC Ag), recombinant human cytokines TNF-∝, GM-CSF, IL-6 and IL-10 (cytokines) or E. coli lipopolysaccharide (LPS). P. vivax paroxysm plasma (PP) was pre-incubated with rabbit serum raised against P. vivax schizonts (IRS-Pv), with rabbit serum raised against P. falciparum schizonts (IRS-Pf) or with convalescent human serum (CHS) collected 4 – 6 weeks after drug cure of a P. vivax infection, from semi-immune individuals resident in a malaria-endemic area prior to being tested in the cell aggregation assay. The table provides the median values together with the interquartile range (25%–75%).