Plasmodium vivax: paroxysm-associated lipids mediate leukocyte aggregation

Malaria Journal

Table 2 Leukocyte aggregation-inducing effects of lipid fractions of Plasmodium vivax paroxysm plasma and of P. vivax schizont extracts with or without supplementation with recombinant human cytokines (TNFα, GM-CSF, IL-6 and IL-10).

Expt. No.	Sample	% R CAI (Median)	25%	75%
1	P1	26.30	20.10	30.60
2	P2	4.50	3.40	8.60
3	P3	4.20	3.50	6.40
4	P4	8.50	6.80	11.80
5	P5	1.00	0.20	1.30
6	P1 + Cytokines	38.20	34.20	46.50
7	P2 + Cytokines	8.70	5.60	10.90
8	P3 + Cytokines	8.60	4.60	12.40
9	P4 + Cytokines	24.60	22.50	31.70
10	P5 + Cytokines	1.20	0.50	3.60
11	S1	3.1	1.4	6.4
12	S2	2.5	1.3	5.9
13	S3	2.9	1.2	7.4
14	S4	14.3	12.3	18.6
15	S5	1	0.2	2.4
16	S1 + Cytokines	10.4	7.6	13.9
17	S2 + Cytokines	3.8	2.5	6.4
18	S3 + Cytokines	2.7	1.9	6.5
19	S4 + Cytokines	26.2	21.9	30.4
20	S5 + Cytokines	1.1	0.3	2.1

% RCAI: % Relative Cell Aggregation Index (median)
25%–75%: 25th and 75th percentiles
Different fractions of P. vivax schizont extracts (S1 – acetone fraction, S2 – ethanol fraction, S3 – petroleum ether fraction, S4 – diethyl ether fraction and S5 – remaining fraction ) and similar fractions of paroxysm plasma ( P1 – acetone fraction, P2 – ethanol fraction, P3 – petroleum ether fraction, P4 – diethyl ether fraction and P5 – remaining fraction ) were tested for their ability to induce cell aggregation with or without supplementation with recombinant human cytokines (TNFα, GM-CSF, IL-6 and IL-10).
The table provides the median values together with the interquartile range (25%–75%).

ISSN: 1475-2875