Figure 3

Agarose gel electrophoresis of deletion mutant AdoMetDC/ODC PCR products. Schematic representations of the wild type (A) (gene size 4257 bp) and the 411 bp deletion mutant (B) (gene size 3846 bp) AdoMetDC/ODC genes inserted into a pASK-IBA3 vector (vector size ~3100 bp). Wild type AdoMetDC/ODC is shown in red, the insert region is in blue, and the deletion mutant AdoMetDC is shown in green. The agarose electrophoresis gel of the deletion mutagenesis PCR products is given in (C) indicating the wild type PCR product of ~7400 bp and the deletion mutants of ~7000 bp produced by the different deletion mutagenesis methods. M) 1 kb marker, 1) wt AdoMetDC/ODC, 2) Overlapping primer PCR product, 3) Inverse PCR product, and 4) RE-mediated inverse PCR product.