Table 2 Deletion mutagenesis efficiency results for the different protocols used. Five clones were analysed for each of the different PCR-based mutagenesis methods based on duplicate PCR experiments.
From: Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study
Primer pair | Mutagenesis method | PCR product analysed with agarose gel electrophoresis | Restriction enzyme mapping with Hind III | Deletion efficiency confirmed with nucleotide sequencing (%) |
|---|---|---|---|---|
P1 | QuickChange™ site-directed method | No product | NA | 0 |
P2 | Overlapping primer method | 7 kb | ~3900 bp ~3100 bp | 40 |
P3 | ExSite™ method | No product | NA | 0 |
Inverse PCR method | 7 kb | ~3900 bp 3100 bp | 0 | |
RE-mediated inverse PCR method | 7 kb | ~3900 bp ~3100 bp | 80 | |
P4 | RE-mediated inverse PCR method | 4.6 kb | Eco RI linearization site removed | 100 |