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Figure 4 | Malaria Journal

Figure 4

From: Malarial pigment haemozoin, IFN-gamma, TNF-alpha, IL-1beta and LPS do not stimulate expression of inducible nitric oxide synthase and production of nitric oxide in immuno-purified human monocytes

Figure 4

iNOS protein expression in (A) immuno-purified human monocytes and (B) RAW264.7 murine macrophages. Cells were fed or not with HZ or sHZ. A portion of the cells was additionally stimulated with IFN-gamma (100 U/ml) or a stimulatory cytokine-LPS mix (MIX+LPS) containing IFN-gamma (400 U/mL), TNF-alpha (500 U/mL), IL-1beta (100 U/mL) and LPS (20 μg/mL) (final concentrations). After 24 h incubation cells were analysed for iNOS expression. Immune-precipitated iNOS from lysate proteins of human monocytes (A) or lysate proteins of RAW264.7 murine macrophages (B) were separated by 10% SDS-PAGE and blotted to PVDF. iNOS was visualized via ECL by binding of a monoclonal anti-iNOS antibody. Recombinant iNOS (Sigma) was used as positive control. Blots shown are representative for five separate experiments.

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Malaria Journal

ISSN: 1475-2875

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