Total number of var transcripts in NF54 and NF54VAR2CSA. Total var transcription is shown as relative copy number for both NF54 and NF54VAR2CSA. Total var transcription of both populations corresponds to the expression of the most abundant transcript, PFL0935c and var2csa (PFL0030c), respectively. The most striking difference in transcription is the slightly earlier distinctive peak for var2csa (PFL0030c) in NF54VAR2CSA compared to the broader wave-like appearance of var transcription in NF54. All measurements at the different time points were normalized against seryl-tRNA synthetase.