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Table 1 Microscopy "long" course training agenda

From: Establishing a malaria diagnostics centre of excellence in Kisumu, Kenya

Day Time Topic
1 9.00 – 9.30 Opening of the session
  9.30 – 10.30 Introduction/Motivation/Goals of the course/Trials and tribulations
  10.45 – 11.45 Clinical presentation of malaria
  11.45 – 12.45 Qualities of a good microscopist
  1.45 – 2.45 Microscope maintenance
  2.45 – 3.45 Standard operating procedure (SOP) development
  4.00 – 5.00 Multiple choice questions (MCQ) written pre-test
  Homework Compare individual SOPs to Walter Reed Project SOPs. Note differences.
2 8.00 – 9.00 Good Clinical Laboratory Practice/Quality control
  9.00 – 10.00 Photographic image pre-test
  10.15 – 1.15 Slide reading pre-test (30 slides: 5 minutes each, 1 minute for passing the slide)
  2.00 – 4.00 Counting pre-test (12 slides: 10 minutes each)
  4.15 – 5.15 Review written test
  Homework Review Bench Aids for the diagnosis of malaria infections
3 8.00 – 10.00 Slide preparation, review form for scoring slide quality
  10.15 – 11.15 Malaria quality assurance & control
  2.00 – 5.00 Laboratory – Slide preparation and staining
4 8.00 – 9.30 Species identification & groups' discussion
  9.45 – 12.45 Laboratory – Species identification
  1.45 – 2.45 Approach to mixed infections
  2.45 – 3.45 Significance of false positives & negatives and counting
  4.00 – 5.00 Review photographic image test with discussion
5 8.00 – 9.30 Artifacts, pseudo-parasites and the reality of false positives
  9.45 – 12.00 Laboratory – Artifacts
  1.00 – 3.45 Laboratory – Slide reading, address individual difficulties
  4.00 – 5.00 SOPs – Groups' presentations and discussions
6, 7 Weekend Open microscopy laboratory
8 8.00 – 9.00 Malaria life cycle
  9.00 – 10.00 Counting basics and techniques
  10.15 – 12.00 Laboratory – Practice counting using Walter Reed Project SOP
  1.00 – 4.30 Laboratory – Compare individual technique results to SOP technique results
  4.45 – 5.15 Review day 1 to 5 microscopy
9 8.00 – 9.00 Low density infections
  9.00 – 9.30 Cross contamination experiment
  9.45 – 5.30 Laboratory – Individual laboratory in reference to the pretest scores
10 8.00 – 9.00 Malaria rereading paradigm
  9.00 – 10.00 Review of the lectures
  10.15 – 12.30 Questions and trouble points, Panel discussion
  1.30 – 5.00 Laboratory – Review and scoring quality of blood slides
11 8.00 – 10.30 Slide reading post-test
  10.45 – 11.45 Written post-test
  11.00 – 12.15 Photographic image post-test
  1.00 – 3.00 Counting post-test
  3.00 – 5.00 Laboratory – Open microscopy and individual help
12 8.00 – 2.00 Go over tests and results, give out take-home training materials