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Table 2 Comparison of fluorescent dyes for dual staining of pRBCs in combination with Giemsa staining.

From: The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria

Fluorescent dye Detection filter Giemsa and fluorescent dye (light/fluorescence)*
SYBR Green 1 fluorescein +/+
Ethidium monoazide (EMA) rhodamine  
   non photoactivated   +/-
   photoactivated   +/-
Yoyo-1 fluorescein +/+
Acridine Orange rhodamine +/-
Ethidium homodimer-2 [EthD-2]) " +/+
SYTO 44 fluorescein +/-
SYTO 45 " +/-
SYTO 17 rhodamine +/-
SYTO 59 " +/-
SYTO 61 " +/-
SYTO 64 " +/-
GelRed " +/-
Hoescht 33258 DAPI +/-
DAPI " +/-
  1. *Plus/minus detection of Giemsa or fluorescence