Table 1 Order-specific group primers and species-specific primers used in blood-meal identification
From: The resting sites and blood-meal sources of Anopheles minimus in Taiwan
Test animal | Primer sequence1 (F: forward 5'-3' and R: reverse 5'-3') | Annealing temp (°C) | Size of amplified products (bp) |
|---|---|---|---|
Avian | F: GACTGTGACAAAATCCCNTTCCA R: GGTCTTCATCTYHGGYTTACAAGAC | 64 | 508 |
Mammalian | F: CGAAGCTTGATATGAAAAACCATCGTTG R: TGTAGTTRTCWGGGTCHCCTA | 59 | 772 |
Chicken | F: GGGACACCCTCCCCCTTAATGACA R: GGAGGGCTGGAAGAAGGAGTG | 69 | 266 |
Bovine | F: GCCATATACTCTCCTTGGTGACA R: GTAGGCTTGGGAATAGTACGA | 61 | 271 |
Pig | F: GCCTAAATCTCCCCTCAATGGTA R: ATGAAAGAGGCAAATAGATTTTCG | 64 | 212 |
Goat | F: TTAAAGACTGAGAGCATGATA R: ATGAAAGAGGCAAATAGATTTTCG | 54 | 225 |
Cat | F: TTCTCAGGATATACCCTTGACA R: GAAAGAGCCCATTGAGGAAATC | 60 | 180 |
Dog | F: GAACTAGGTCAGCCCGGTACTT R: CGGAGCACCAATTATTAACGGC | 67 | 153 |
Horse | F: CCCTAAGCCTCCTAATCCGT R: AGGAATGATGGGGGAAGTAA | 56 | 235 |
Human | F: TTCGGCGCATGAGCTGGAGTCC R: TATGCGGGGAAACGCCATATCG | 70 | 228 |
Monkey | F: CCTCTTTCCTGCTGCTAATG R: TTTGATACTGGGATATGGCG | 62 | 222 |
Rat | F: CGGCCACCCAGAAGTGTACATC R: GGCTCGGGTGTCTACATCTAGG | 67 | 196 |