Table 1 Order-specific group primers and species-specific primers used in blood-meal identification
From: The resting sites and blood-meal sources of Anopheles minimus in Taiwan
| Test animal | Primer sequence1 (F: forward 5'-3' and R: reverse 5'-3') | Annealing temp (°C) | Size of amplified products (bp) |
|---|---|---|---|
| Avian |
F: GACTGTGACAAAATCCCNTTCCA R: GGTCTTCATCTYHGGYTTACAAGAC | 64 | 508 |
| Mammalian |
F: CGAAGCTTGATATGAAAAACCATCGTTG R: TGTAGTTRTCWGGGTCHCCTA | 59 | 772 |
| Chicken |
F: GGGACACCCTCCCCCTTAATGACA R: GGAGGGCTGGAAGAAGGAGTG | 69 | 266 |
| Bovine |
F: GCCATATACTCTCCTTGGTGACA R: GTAGGCTTGGGAATAGTACGA | 61 | 271 |
| Pig |
F: GCCTAAATCTCCCCTCAATGGTA R: ATGAAAGAGGCAAATAGATTTTCG | 64 | 212 |
| Goat |
F: TTAAAGACTGAGAGCATGATA R: ATGAAAGAGGCAAATAGATTTTCG | 54 | 225 |
| Cat |
F: TTCTCAGGATATACCCTTGACA R: GAAAGAGCCCATTGAGGAAATC | 60 | 180 |
| Dog |
F: GAACTAGGTCAGCCCGGTACTT R: CGGAGCACCAATTATTAACGGC | 67 | 153 |
| Horse |
F: CCCTAAGCCTCCTAATCCGT R: AGGAATGATGGGGGAAGTAA | 56 | 235 |
| Human | F: TTCGGCGCATGAGCTGGAGTCC R: TATGCGGGGAAACGCCATATCG | 70 | 228 |
| Monkey |
F: CCTCTTTCCTGCTGCTAATG R: TTTGATACTGGGATATGGCG | 62 | 222 |
| Rat | F: CGGCCACCCAGAAGTGTACATC R: GGCTCGGGTGTCTACATCTAGG | 67 | 196 |