Comparison of the bead-based assay and ELISA. Four recombinant PfEMP1 proteins (C17, D5, D9 and D12) were analysed by ELISA and by the bead-based assay using twenty plasma samples from Danish donors and plasma samples from 60 individuals living in the three Tanzania villages Mgome (high malaria transmission), Ubiri (medium malaria transmission) and Magamba (low malaria transmission). The samples for both assay platforms were diluted 1:80 and results were expressed as median fluorescent intensity for the bead-based assay and arbitrary ELISA Units for ELISA. The Spearman's rank correlation coefficients (Rho) were ≥ 0.86, (P < 0.0001). Note: The graphs have different scales. MFI: median fluorescent intensity and EU: ELISA units.