Table 4 Selected studies investigating Hz-containing leukocytes and disease severity
Reference year published | Mujuzi et al. 2006 [32] | Lyke et al. 2003 [9] | Amodu et al. 1998 [8] | Phu et al. 1995 [31] | Metzger et al. 1995 [7] |
|---|---|---|---|---|---|
place of study/endemicity | Northern Uganda intense holoendemic | Mali/intense seasonal | Nigeria/holoendemic | Viet Nam/ND | Gabon/hyperendemic |
population | children, 6–59 months old | children, 3 months to 14 years old | children | adults | children, 6 months to 14 years old |
study size | (n = 208), severe (n = 99)/uncomplicated (n = 99) | (n = 516), severe (n = 163), uncomplicated (n = 163), healthy (n = 164) | (n = 146), cerebral (n = 43), uncomplicated (n = 43), asymptomatic (n = 32), no malaria (n = 28) | (n = 300), cerebral (n = 168), fatal (n = 40) | (n = 73), severe (n = 42), mild (n = 31) |
WBC count method | assuming 8,000/μL | assuming 7,500/μL | assuming 8,000/μL | assuming 8,000/μL | ND |
Hz-leucocyte counting method | thin film/counting 500 leukocytes | thin films/counting 30 monocytes, 100 PCN | thick films/counting 30 monocytes, 100 PCN | thin & thick films/counting 30 monocytes, 100 PCN | thick films/counting 100 monocytes, 100 PCN |
quality assessment of microscopy | ND | 10% of slides re-examined by 2nd micoscopist | ND | 3 slides observed by 10 microscopists/1 slide examined 10× times by the same microscopist | count independently by two investigators |
Result of quality assessment | ND | Kappa coefficient for: – PMN: 0,88 – mono: 0,77 | ND | -mono: 125, 76 and 40 | ND |