Localization of SURFIN
by immunofluorescence staining on air-dried monolayers from 3D7S8 parasite strain. Air dried monolayers probed with rabbit anti-SURFIN4.1 on 3D7S8 pRBC. Propidium iodide (red) was used to stain the parasite nucleus and SURFIN4.1 and SURFIN4.2 proteins were stained green using anti-rabbit Alexa 488. SURFIN4.1 localizes within the parasitophorous vacuole (PV) and is observed from approximately 30 hrs post invasion. SURFIN4.1 was observed as a green dot above the food vacuole (a), at 30–35 hrs parasite stages. The protein was spread around the parasitophorous vacuole (PV) at 35–40 hrs parasite stages and in the mature schizont (44–48 hrs) SURFIN4.1was observed between the dividing merozoites. During the trophozoite and early schizont stages SURFIN4.2 shows a similar pattern of staining as SURFIN4.1.