Figure 1
From: Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
Schematic representation of the MSP-1 and MSP-1/MSP-6/MSP-7 complex antigens used in the study. The schematic representation of the MSP-1 protein is shown (A). The MSP-1 precursor protein (i) undergoes proteolytic cleavage into four subunits as shown (ii). The MSP-142 molecule is further cleaved to MSP-133 and MSP-119 (iii). A proposed model (adapted from [23]) demonstrating the interactions of MSP-1 protein with the MSP-636 and MSP-722 molecules (B). The two allelic forms of MSP-183, MSP-130, MSP-138 and MSP-142 (D and F), in addition to MSP-636, MSP-722 and MSP-119, were expressed in E. coli and purified [49, 50]. The purity of these recombinant proteins was examined by using 12% SDS-PAGE followed by Coomassie staining (C). Molecular weight (MW) is shown in kDa.