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Table 6 Stability Study – Loss relative to -20°C Sample (Log10 drop)

From: Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT)-based assays

Months Storage

Storage Temperature

-20°C

+4°C

+20°C

+37°C

+45°C

8

0.00

-0.04

0.26

0.41

0.44

12

0.00

0.06

0.35

0.62

0.62

  1. The accelerated thermal degradation samples were stored at +4°C, +20°C, +37°C and 45°C for a period of 8 and 12 months, these samples were compared to vials of AA that were stored continuously at -20°C. After 8 months, six vials of each sample stored at the elevated temperatures were analysed, with all sample extracts being tested singly. After 12 months, two vials of each sample stored at the elevated temperatures were analysed, with all sample extracts being tested duplicate. Mean values are shown for the log10 drop in titre.