Var transcription profiling of Plasmodium falciparum 3D7: assignment of cytoadherent phenotypes to dominant transcripts

Table 1 Cytoadherence of differently selected 3D7 parasites selected for adhesion on the indicated CHO-cell lines

	CHO-745	CHO-K1	CHO-CD36	CHO-ICAM	CHO-Sel	CHO-VCAM
3D7	7 ± 2	0	12 ± 4	7 ± 3	5 ± 1	4 ± 2
3D7-745	142 ± 13	120 ± 23	15 ± 3	253 ± 22	28 ± 5	270 ± 26
3D7-K1	25 ± 3	29 ± 5	32 ± 8	45 ± 8	45 ± 3	34 ± 4
3D7-CD36*	16 ± 3	3 ± 1	62 ± 7	33 ± 5	25 ± 5	23 ± 6
3D7-ICAM*	40 ± 9	40 ± 7	152 ± 12	235 ± 18	141 ± 13	151 ± 17
3D7-Selectin	13 ± 2	16 ± 3	21 ± 5	50 ± 8	34 ± 3	15 ± 5
3D7-VCAM	51 ± 11	51 ± 8	92 ± 12	226 ± 21	151 ± 14	51 ± 5

A stock of freshly panned parasites was thawed, put into culture and after 4–6 reinvasions, 5*10⁶ IRBC (at trophozoite stage) were incubated on the indicated CHO-cell lines, washed and counted. For each cell line, 1000 cells and the adhering parasites were counted and cytoadherence was expressed as IRBC per 100 cells. Phenotypes which significantly (Chi-square method, p < 0,05) adhered more to the CHO cell line for which they were selected than to any other cell line are marked with an asterisk.

ISSN: 1475-2875