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Figure 5 | Malaria Journal

Figure 5

From: Phagocytosis of haemozoin (malarial pigment) enhances metalloproteinase-9 activity in human adherent monocytes: Role of IL-1beta and 15-HETE

Figure 5

IL-1beta mRNA expression in immunopurified human adherent monocytes unfed or fed with HZ and treated or not with 15-HETE. Human CD14+ immunopurified monocytes were unfed, fed with HZ or treated with 15-HETE added at time 0 at 1–10 μM (final concentration). 6 h after phagocytosis or addition of 15-HETE. mRNA expression was measured by real-time quantitative RT-PCR in cell lysates and expressed as -fold variation over untreated monocytes. Mean values ± SD of three independent experiments. Data were analysed for significance by Student's t-test. Significance of differences (column numbers). Unfed(1) vs HZ-fed(2) monocytes, p < 0.01; unfed(1) vs 15-HETE 1 μM(3) treated monocytes, n.s.; unfed(1) vs 15-HETE 10 μM(4) treated monocytes, p < 0.05.

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Malaria Journal

ISSN: 1475-2875

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